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普诺赛HS 683 (人脑胶质瘤细胞)

品名: 普诺赛HS 683 (人脑胶质瘤细胞)
型号: CL-0362
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产品信息

名称HPAC (人胰腺腺泡上皮癌)(暂不出售)
别称Hpac
种属
年龄(性别)64岁
组织来源胰腺
生长特性贴壁细胞
细胞形态上皮细胞样
细胞类型肿瘤细胞
肿瘤类型胰腺癌细胞
生物安全等级1
生长培养基DMEM/F12+2μg/ml Insulin+5μg/ml Transferrin+40ng/ml Hydrocortisone+10ng/ml EGF+5% FBS1% P/S
推荐传代比例1:3-1:4
推荐换液频率2~3次/周
倍增时间~23-44 hours
冻存条件冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮
培养条件气相:空气,95%;CO2,5%温度:37℃
致瘤性Yes, the cells form tumors in athymic nude mice at the site of inoculation which are histologically similar to the tumor of origin. Yes, the cells have a colony forming efficiency of 64% on plastic and 3.2% in agarose.
受体表达情况epidermal growth factor (EGF), expressed; glucocorticoid, expressed; epidermal growth factor (EGF); glucocorticoid
基因表达情况HPAC cells are positive for keratin and negative for vimentin and chromogranin A.
保藏机构ATCC; CRL-2119


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