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普诺赛SNB-19 (人胶质瘤细胞)

品名: 普诺赛SNB-19 (人胶质瘤细胞)
型号: CL-0582
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产品信息

名称SNB-19 (人胶质瘤细胞) (U-251 MG污染细胞系,暂不供应)
别称SNB.19; SNB19
背景描述Established from the surgical resection of a left parieto-occipital glioblastoma from a 47-year-old man in 1980; cells were described in the literature to secrete plasminogen activator, to be clonogenic in soft agar and to be tumorigenic in nude mice; DNA fingerprinting showed unequivocally that SNB-19 is a subclone of the astrocytoma cell line U-251 MG)。(STR检测位点同U-251MG)
种属
年龄(性别)男;75岁
组织来源星形胶质瘤(U-251MG)
生长特性贴壁细胞
细胞形态成纤维细胞样
细胞类型肿瘤细胞
肿瘤类型胶质瘤细胞
生物安全等级1
生长培养基DMEM10% FBS1% P/S
推荐传代比例1:4-1:6
推荐换液频率2~3次/周
倍增时间~24-36 hours
冻存条件冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮
培养条件气相:空气,95%;CO2,5%温度:37℃
保藏机构DSMZ; ACC-325


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