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普诺赛HTR-8/Svneo (人绒毛膜滋养层细胞)

品名: 普诺赛HTR-8/Svneo (人绒毛膜滋养层细胞)
型号: CL-0599
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产品信息

名称HTR-8/Svneo (人绒毛膜滋养层细胞) (暂不出售)
别称HTR-8/SV neo; HTR-8/SV-neo; HTR8/SVneo; HTR8svn
背景描述The HTR-8/SVneo cell line was derived by transfecting the cells that grew out of chorinic villi explants of human first-trimester placenta with the gene encoding for simian virus 40 large T antigen.
种属
年龄(性别)女;6-12周胚胎
组织来源胎盘
生长特性贴壁细胞
细胞形态上皮细胞样
细胞类型转化细胞系
生物安全等级2
生长培养基RPMI-16405% FBS1% P/S
推荐传代比例1:3-1:5
推荐换液频率2~3次/周
冻存条件冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮
培养条件气相:空气,95%;CO2,5%温度:37℃
致瘤性No (tested in soft agar and nude mice).
抗原表达情况cytokeratin-7+; insulin-like growth factor (IGF)-II+; NDOG-5 (placental alkaline phosphatase (PLAP) antibody) +; proliferating cell nuclear antigen (PCNA)+; human leukocyte antigen framework antigen (W6/32)+; integrin subunits alpha 1, alpha 3, alpha
保藏机构ATCC; CRL-3271


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