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普诺赛KYSE-150 (人食管鳞癌细胞)

品名: 普诺赛KYSE-150 (人食管鳞癌细胞)
型号: CL-0638
  • 产品详情
  • 规格参数

产品信息

名称KYSE-150 (人食管鳞癌细胞) (STR鉴定正确)
别称KYSE 150; KYSE150; Kyse150; KY150
背景描述Established from the poorly differentiated esophageal squamous cell carcinoma resected from upper (cervical) esophagus of a 49-year-old Japanese woman after receiving radiotherapy (tumor was invading contiguous structures); described as carrying amplified oncogenes, c-erb-B (8x) and cyclin D1 (4x) and producing tumors in nude mice.
种属
年龄(性别)女;49岁
组织来源食管
生长特性贴壁细胞
细胞形态上皮细胞样
细胞类型肿瘤细胞
肿瘤类型食管癌细胞
生物安全等级1
生长培养基RPMI-1640Ham's F-1210% FBS1%P/S
推荐传代比例1:4-1:8
推荐换液频率2~3次/周
倍增时间13.7 hours (PubMed=1728357); 23 hours (PubMed=25984343); <25 hours, less when serum amount is increased (DSMZ)
冻存条件冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮
培养条件气相:空气,95%;CO2,5%温度:37℃
致瘤性Yes, in nude mice
保藏机构DSMZ; ACC-375
STR鉴定
  • STR位点信息

    AmelogeninX
    CSF1PO12,13
    D2S133825
    D3S135815,16
    D5S81812,13
    D7S82010,11
    D8S117910,15
    D13S3178,11
    D16S5399,11
    D18S5114
    D19S43315,15.2
    D21S1130,31
    FGA21,24
    PentaD10
    PentaE12,18
    TH017,9
    TPOX8
    vWA16,17
    D6S104318,20
    D12S39119,22
    D2S44110,11


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