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普诺赛SU-DHL-4 (人B淋巴瘤细胞)

品名: 普诺赛SU-DHL-4 (人B淋巴瘤细胞)
型号: CL-0692
  • 产品详情
  • 规格参数

产品信息

名称SU-DHL-4 (人B淋巴瘤细胞) (STR鉴定正确)
别称SUDHL4; Sudhl4; SUDHL-4; Sudhl-4; SuDHL 4; SUD-4; SUD4; SU4; Stanford University-Diffuse Histiocytic Lymphoma-4; DHL-4; DHL4
背景描述Established from the peritoneal effusion of a 38-year-old man with B-NHL (diffuse large cell, cleaved cell type; originally described as "diffuse histiocytic lymphoma") in 1975; cell line carries EZH2 Y641S mutation; assigned to GCB-like lymphoma subtype (germinal center B-cell). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)
种属人类
年龄(性别)男性,38岁
组织来源腹腔积液
生长特性悬浮细胞
细胞形态淋巴母细胞样
细胞类型肿瘤细胞
肿瘤类型淋巴瘤细胞
生长培养基RPMI-164010% FBS1% P/S
推荐传代比例5×10^5-1×10^6cells/mL
推荐换液频率2~3次/周
倍增时间~40小时
冻存条件冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮
培养条件气相:空气,95%;CO2,5%温度:37℃
基因表达情况IgG+; Kappa+; IgM-; IgA-; IgD-; Lambda-; This cell line has relatively high expression levels of Bax; Bak; AIF; high caspase-9 activity.
注意事项该细胞为悬浮细胞,请注意离心收集细胞悬液;请勿直接倒掉细胞培养液。
保藏机构ATCC; CRL-2957 DSMZ; ACC-495
STR鉴定
  • STR位点信息

    AmelogeninX,Y
    CSF1PO12
    D2S133817,19
    D3S135815,16
    D5S81811,12
    D7S8208,11
    D8S117913
    D13S31712
    D16S53911,13
    D18S5114,16
    D19S43314,15.2
    D21S1129,31
    FGA19,21
    PentaD9,14
    PentaE7,12
    TH016,9.3
    TPOX9,11
    vWA18,19
    D6S104311
    D12S39118,20
    D2S44111,14


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