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普诺赛9L/lacZ (大鼠胶质肉瘤细胞)

品名: 普诺赛9L/lacZ (大鼠胶质肉瘤细胞)
型号: CL-0637
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产品信息

名称9L/lacZ (大鼠胶质肉瘤细胞) (种属鉴定正确)
别称9L/LacZ
背景描述The cells constitutively express the lacZ reporter gene product, E. coli derived beta-gal, as revealed on tissue sections by histochemical stain, and single tumor cells can be identified. Lymphocytes and other responding cells can be identified by double labeling with antibodies on the same slide. The contrast between stained cells and background facilitates image analysis. The beta-gal expression is very stable, but cells may need to be re-cloned after months of growth in culture.
种属大鼠
年龄(性别)雄性
组织来源脑,胶质细胞
生长特性贴壁细胞
细胞形态成纤维细胞样
细胞类型肿瘤细胞
肿瘤类型胶质瘤细胞
生物安全等级1
生长培养基DMEM10%FBS1%P/S
推荐传代比例1:4-1:8
推荐换液频率2~3次/周
冻存条件冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮
培养条件气相:空气,95%;CO2,5%温度:37℃
致瘤性Yes, forms tumors in the brains of CD Fischer 344 rats
基因表达情况beta galactosidase (beta-gal)
保藏机构ATCC; CRL-2200


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